weissman genome wide crispr activation crispra library  (Addgene inc)

Journal: bioRxiv

Article Title: Systematic Evaluation Defines the Limits of Ferroptosis in Cancer Therapy

doi: 10.64898/2026.03.11.711115

Figure Lengend Snippet: A , Immunoblot from lysates of A549 cells or HT-1080 cells for indicated proteins in the presence or absence of CySS or βMe (50μM). Inferred MW of band indicated. B , Viability (ATP) of HT-1080 or KYSE30 cells expressing the indicated GPX4 cDNAs and selenocysteine (U46C) or SEICS (Δ3’UTR) mutants, cultured in indicated CySS conditions, 3 days. Data report mean ± SEM of n=3 biological replicates. C , Model-based Analysis of Genome-scale CRISPR-Cas9 Knockout (MAGeCK) score from genome-wide CRISPR screens of A549 SLC7A11 knockout cells or A549 cells cultured in the absence of cystine comparing culture in the absence or presence of βMe (50μM). LPCAT3 (red dot, indicated) is the top ranked gene in both conditions for promoting survival in the absence of βMe. Data are from a single screen. D , Viability (ATP) of indicated cell lines cultured in indicated CySS conditions or RSL-3 dose, in combination with inhibitors of ACSL4 (ROSI, 25μM) or LPCAT3 (HTS-3, 10μM), 3 days. Data report mean ± SEM of n=3 biological replicates.

Article Snippet: Genome-wide resistance screens were performed using the Human Activity-Optimized CRISPR Knockout Library (Addgene pooled library #1000000100).

Techniques: Western Blot, Expressing, Cell Culture, CRISPR, Knock-Out, Genome Wide